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医学生物化学与分子生物学实验指南(第2版)(英文版)

  • 定价: ¥33
  • ISBN:9787307211674
  • 开 本:16开 平装
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  • 折扣:
  • 出版社:武汉大学
  • 页数:201页
  • 作者:编者:喻红//陈娟
  • 立即节省:
  • 2019-11-01 第2版
  • 2019-11-01 第1次印刷
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导语

  

内容提要

  

    本书难易适中、内容创新实用,主要适用对象为医学留学生(本科及研究生),以及我国医学长学制硕博研究生。本书在上一版基础上增加了分子生物前沿技术发展及在医学研究中现代常用技术的内容;对生物化学实验技术基础理论与经典实验部分进行了修订,介绍基本操作技术、分光光度法、电泳技术、层析技术的原理及经典实验,并以核酸、蛋白质为对象,介绍研究基础实验的原理和应用;凝练了综合性实验实践,以酶学分析、蛋白质分离纯化鉴定、核酸分离及鉴定、基因工程为系统性实验内容;新增了医学创新实验设计与应用,以生物大分子的结构与功能研究为对象,结合医学学科研究特点,提出引导问题,为学生开展自主创新实验的设计与应用提供设计流程、模拟报告的指导。

目录

Section Ⅰ Theories and Strategies of Biochemistry and
  Molecular Biology Technology in Medical Research
Chapter 1 Medical Application of Biochemistry and Molecular Biology Technology
  1.1  Basic Objectives of Biochemistry and Molecular Biology Technology
  1.2  Application of Biochemistry and Molecular Biology Technology in Medical Research
Chapter 2 The Common Research Techniques of Gene
  2.1  Nucleic Acid Hybridization and DNA Microarray
  2.2  DNA Sequencing
  2.3  Polymerase Chain Reaction (PCR)
  2.4  Recombinant DNA Technology
  2.5  The Research Strategies and Methods for Gene Transcription Regulation
Chapter 3 The Common Research Techniques of Protein
  3.1  Quantification and Identification of Protein
  3.2  Methods and Basic Principles of Protein Purification
  3.3  Protein Chemical Modification and Spatial Structure Analysis
  3.4  Protein Interaction Research Methods
  3.5  Proteomies Research
  3.6  Research Technology of Enzymology
Section Ⅱ Basic Biochemical Techniques
Chapter 4 Basic Operation of Biochemical Experiments
  4.1  Solution Preparation
  4.2  Pipettes Operation
  4.3  Centrifuge Operation
Chapter 5 Spectrophotometry
  5.1  Basic Concepts and Principle of Spectrophotometry
  5.2  Measurement of Light Absorption
  5.3  Applications of Spectrophotometry
  Experiment 1 Quantitative Analysis of Protein
  Experiment 2 Determination of Blood Glucose Level Regulated by Hormones
Chapter 6 Electrophoresis
  6.1  Basic Principles of Electrophoresis
  6.2  Impact Factors of Electrophoresis
  6.3  Detection of Components after Electrophoretic Separation
  6.4  Special Electrophoretic Techniques and Applications
  Experiment 3 Separation of Serum Proteins by Cellulose Acetate Membrane Electrophoresis
  Experiment 4 Separation of Serum Lipoproteins by Agarose Gel Electrophoresis
  Experiment 5 Proteins Separation by SDS-Polyacrylamide Gel Electrophoresis
Chapter 7 Chromatography
  7.1  Fundamental Principles of Chromatography
  7.2  Commonly Used Chromatographic Techniques
  Experiment 6 Separation of Mixed Amino Acids by Cation Exchange Chromatography
  Experiment 7 Separation of Hemoglobin and Dinitrophenyl (DNP) -glutamate by Gel Filtration Chromatography
Section Ⅲ Integrative Experiments
Chapter 8 Enzyme Analysis
  8.1  The Activity of Enzyme
  8.2  Enzyme Kinetics ( Factors Affecting Reaction Velocity)
  8.3  Enzymatic Analysis
  Experiment 8 Assay the Activity of Alanine Aminotransferase (ALT) in Serum ( Mohun's Method)
  Experiment 9 Effect of Substrate Concentration on Enzyme Activity-Determining Km Value for Alkaline Phosphatase
  Experiment 10 Enzymatic Endpoint Method-Determination of Total Cholesterol, Triglycerides and Glucose Levels in Serum
Chapter 9 Isolation, Purification and Identification of Protein
  9.1  Procedure of Protein Isolation
  9.2  The Selective Purification Steps
  9.3  Crystallization, Concentration, Drying and Storage of Protein
  9.4  Identification and Analysis of Protein
  Experiment 11 Isolation and Identification of Serum IgG
  Experiment 12 Purification and Identification of Glutathione S-Transferase Fusion Protein
Chapter 10 Isolation, Purification and Identification of Nucleic Acids
  10.1  Preparation Principle and Procedure of Nucleic Acids
  10.2  Identification and Analysis of Nucleic Acids
  Experiment 13 Isolation and Identification of Eukaryotic Genomie DNA
  Experiment 14 SDS-Alkaline Lysis Preparation of Plasmid DNA
  Experiment 15 Isolation and Identification of Total RNA in Tissues/Cells
  Experiment 16 Polymerase Chain Reaction (PCR) and Reverse Transeription-PCR
Chapter 11 Genetic Engineering
  11.1  The Process of Genetic Engineering
  11.2  Expression of Foreign Genes and Purification of Recombinant Proteins
  Experiment 17 DNA Cloning
  Experiment 18 Expression of Exogenous Gene in E.eoli and Purification of His-tag Proteins
Chapter 12 Innovation Experiment Design and Application
  Experiment 19 Separation and Identification of Alkaline Phosphatase
  Experiment 20 Lactate Dehydrogenase (LDH) Analysis
  Experiment 21 Protein Markers Exploration by Two-dimensional Eleetrophoresis
Reference